Analysis of Epstein-Barr viral genomes in lymphoid malignancy using Southern blotting, polymerase chain reaction and in situ hybridization

109 malignant lymphomas were surveyed by Southern blot analysis and polymerase chain reaction (PCR) for Epstein-Barr virus (EBV) DNA and compared with 16 examples of non-neoplastic lymphadenopathy and 4 normal thymuses. In specimens positive by the method of Southern and PCR, in situ hybridization s...

Full description

Saved in:
Bibliographic Details
Published in:Virchows Archiv. B, Cell pathology Cell pathology, 1990, Vol.59 (6), p.383-390
Main Authors: Ohshima, K, Kikuchi, M, Eguchi, F, Masuda, Y, Sumiyoshi, Y, Mohtai, H, Takeshita, M, Kimura, N
Format: Article
Language:English
Subjects:
Base Sequence
DNA Probes
DNA, Viral - analysis
Gene Rearrangement
Gene Rearrangement, T-Lymphocyte
Herpesvirus 4, Human - genetics
Hodgkin Disease - microbiology
Hodgkin Disease - pathology
Humans
Immunoglobulin Heavy Chains - genetics
Lymphadenitis - microbiology
Lymphoma - microbiology
Lymphoma, B-Cell - microbiology
Lymphoma, B-Cell - pathology
Lymphoma, T-Cell - microbiology
Lymphoma, T-Cell - pathology
Molecular Sequence Data
Nucleic Acid Hybridization
Polymerase Chain Reaction
Thymus Gland - microbiology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:109 malignant lymphomas were surveyed by Southern blot analysis and polymerase chain reaction (PCR) for Epstein-Barr virus (EBV) DNA and compared with 16 examples of non-neoplastic lymphadenopathy and 4 normal thymuses. In specimens positive by the method of Southern and PCR, in situ hybridization studies were performed on formalin-fixed, paraffin-embedded sections. By Southern blot analysis, two of seven Hodgkin's disease samples (29%) (one of mixed cellularity and the other of lymphocyte predominance type), three of 56 B-cell lymphomas (5.6%) and five of 46 T-cell lymphomas (11%) demonstrated EBV DNA. However, the 16 examples of lymphadenitis and the 4 normal thymuses showed no EBV DNA. With PCR, EBV DNA was identified in one B-cell lymphoma, nine T-cell lymphomas, ten lymphadenitis specimens and two of the normal thymus, in addition to the positive specimens determined by the Southern blotting method. These results indicate that the presence of EBV DNA is not related to lymphoid malignancy, but enhancement of the DNA is demonstrated in some neoplastic conditions. By in situ hybridization, EBV genomes were not detected in all PCR-positive cases, but only in those positive by Southern blot analysis.
ISSN:0340-6075