A quantitative assessment of F-actin content and distribution in untreated and butyric acid treated murine melanoma B16a tumour cells : a fluorescence image analysis study

Image analysis of phallacidin, a fluorescent stoichiometric probe to F-actin, permitted the cytoskeletal-associated actin 'F-actin' to be visualized morphologically and to be divided into two groups, diffuse and filamentous. The filamentous actin group was categorized further into four sub...

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Bibliographic Details
Published in:The Histochemical journal 1990-11, Vol.22 (11), p.624-627
Main Authors: PERSKY, B, MCGARVEY, T
Format: Article
Language:English
Subjects:
Actins - metabolism
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Butyrates - metabolism
Butyrates - pharmacology
Butyric Acid
Contractile proteins
Fluorescence
Fundamental and applied biological sciences. Psychology
Holoproteins
Image Processing, Computer-Assisted
Melanoma, Experimental - metabolism
Melanoma, Experimental - pathology
Mice
Peptides, Cyclic - metabolism
Proteins
Tumor Cells, Cultured
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Summary:Image analysis of phallacidin, a fluorescent stoichiometric probe to F-actin, permitted the cytoskeletal-associated actin 'F-actin' to be visualized morphologically and to be divided into two groups, diffuse and filamentous. The filamentous actin group was categorized further into four subgroups according to the intensity of the phallacidin probe. F-actin groups and subgroups of untreated cells and cells treated with 1.5 mM butyrate acid were analysed independently. Butyric acid treatment significantly increased total actin, defined as diffuse actin, plus filamentous subgroup actins 1-4. Specifically, butyric acid-treatment increased filamentous subgroup actin 1.
ISSN:0018-2214
1573-6865
DOI:10.1007/BF01072944