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A quantitative assessment of F-actin content and distribution in untreated and butyric acid treated murine melanoma B16a tumour cells : a fluorescence image analysis study

Image analysis of phallacidin, a fluorescent stoichiometric probe to F-actin, permitted the cytoskeletal-associated actin 'F-actin' to be visualized morphologically and to be divided into two groups, diffuse and filamentous. The filamentous actin group was categorized further into four sub...

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Published in:	The Histochemical journal 1990-11, Vol.22 (11), p.624-627
Main Authors:	PERSKY, B, MCGARVEY, T
Format:	Article
Language:	English
Subjects:	Actins - metabolism Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Butyrates - metabolism Butyrates - pharmacology Butyric Acid Contractile proteins Fluorescence Fundamental and applied biological sciences. Psychology Holoproteins Image Processing, Computer-Assisted Melanoma, Experimental - metabolism Melanoma, Experimental - pathology Mice Peptides, Cyclic - metabolism Proteins Tumor Cells, Cultured
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container_title	The Histochemical journal
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description	Image analysis of phallacidin, a fluorescent stoichiometric probe to F-actin, permitted the cytoskeletal-associated actin 'F-actin' to be visualized morphologically and to be divided into two groups, diffuse and filamentous. The filamentous actin group was categorized further into four subgroups according to the intensity of the phallacidin probe. F-actin groups and subgroups of untreated cells and cells treated with 1.5 mM butyrate acid were analysed independently. Butyric acid treatment significantly increased total actin, defined as diffuse actin, plus filamentous subgroup actins 1-4. Specifically, butyric acid-treatment increased filamentous subgroup actin 1.
doi_str_mv	10.1007/BF01072944
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Psychology</subject><subject>Holoproteins</subject><subject>Image Processing, Computer-Assisted</subject><subject>Melanoma, Experimental - metabolism</subject><subject>Melanoma, Experimental - pathology</subject><subject>Mice</subject><subject>Peptides, Cyclic - metabolism</subject><subject>Proteins</subject><subject>Tumor Cells, Cultured</subject><issn>0018-2214</issn><issn>1573-6865</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNpFkU9v1DAQxS0EKkvhwh3JF3pACvWfbOxwa6suIFXqBc7RxJ4go8RuPTbSfia-JFm6bU8jze_p6c08xt5L8VkKYc4vd0IKo_q2fcE2cmt009lu-5JthJC2UUq2r9kbot9CiN6Y7oSdKGFaJcWG_b3g9xViCQVK-IMciJBowVh4mviuAVdC5C7FclhB9NwHKjmMtYQU-cpqLBmhoP9P1_0-B8fBBc8fwVJziMgXnCGmBfil7ICXuqSaucN5Jv6FA5_mmjKSw-iQhwV-rWkizHsKxKlUv3_LXk0wE747zlP2c3f94-pbc3P79fvVxU3jtFSlsVpbh1YqC6prNXZ2HI2aWqM8aNy2YuxV56YRPFjf2tZ6FM55O-mxG9eP6VN29uB7l9N9RSrDEuiQEyKmSoMVypit7Vbhpwehy4ko4zTc5TV43g9SDIdmhudmVvGHo2sdF_RP0mMVK_945EAO5ilDdIGeHfv1ACV7_Q8FHZiw</recordid><startdate>19901101</startdate><enddate>19901101</enddate><creator>PERSKY, B</creator><creator>MCGARVEY, T</creator><general>Kluwer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19901101</creationdate><title>A quantitative assessment of F-actin content and distribution in untreated and butyric acid treated murine melanoma B16a tumour cells : a fluorescence image analysis study</title><author>PERSKY, B ; MCGARVEY, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c312t-8338ce8128a2643e68bb72f472da3e540b926cfbada8d4848de0ccd8f3b6b0013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Actins - metabolism</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Butyrates - metabolism</topic><topic>Butyrates - pharmacology</topic><topic>Butyric Acid</topic><topic>Contractile proteins</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. 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subjects	Actins - metabolism Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Butyrates - metabolism Butyrates - pharmacology Butyric Acid Contractile proteins Fluorescence Fundamental and applied biological sciences. Psychology Holoproteins Image Processing, Computer-Assisted Melanoma, Experimental - metabolism Melanoma, Experimental - pathology Mice Peptides, Cyclic - metabolism Proteins Tumor Cells, Cultured
title	A quantitative assessment of F-actin content and distribution in untreated and butyric acid treated murine melanoma B16a tumour cells : a fluorescence image analysis study
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