A Rapid, Quantitative GLC Method for the Simultaneous Determination of Nicotine and Cotinine

Published gas-liquid chromatographic (GLC) methods for the determination of nicotine and cotinine have proved impractical for the analysis of a large number of clinical samples. Significant improvements over other methods have been achieved, being low sample volume (0.5 mL plasma), rapid two-step ex...

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Bibliographic Details
Published in:Journal of analytical toxicology 1982-11, Vol.6 (6), p.294-296
Main Authors: Verebey, Karl G., DePace, Ann, Mule, S. Joseph, Kanzler, Maureen, Jaffe, Jerome H.
Format: Article
Language:English
Subjects:
Chromatography, Gas
Cotinine
Cotinine - blood
Evaporation
Humans
Nicotine
Nicotine - blood
Pyrrolidinones - blood
Smoking
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Summary:Published gas-liquid chromatographic (GLC) methods for the determination of nicotine and cotinine have proved impractical for the analysis of a large number of clinical samples. Significant improvements over other methods have been achieved, being low sample volume (0.5 mL plasma), rapid two-step extraction from plasma, no evaporation step, and good separation. The lower limits of sensitivity for nicotine and cotinine were 1 and 5 ng/mL, respectively. The method was validated by the analysis of plasma samples from cigarette-smoking volunteers. The method described permits the quick, routine determination of nicotine and cotinine in a large number of samples.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/6.6.294