Development of an ELISA to study the polymorphism of dextromethorphan oxidation in a french population

Simple, sensitive and selective enzyme immunoassays (ELISA) for monitoring urinary dextromethorphan and its major metabolite, dextrorphan, were developed. Dextromethorphan and dextrorphan hemisuccinates were linked to bovine serum albumin and specific antisera against each immunogen were raised in r...

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Bibliographic Details
Published in:European journal of clinical pharmacology 1990-01, Vol.39 (5), p.481-485
Main Authors: FRECHE, J. P, DRAGACCI, S, PETIT, A. M, SIEST, J. P, GALTEAU, M. M, SIEST, G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromatography, High Pressure Liquid
Dextromethorphan - metabolism
Enzyme-Linked Immunosorbent Assay - methods
Female
France
General pharmacology
Humans
Male
Medical sciences
Oxidation-Reduction
Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions
Pharmacology. Drug treatments
Phenotype
Polymorphism, Genetic
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Summary:Simple, sensitive and selective enzyme immunoassays (ELISA) for monitoring urinary dextromethorphan and its major metabolite, dextrorphan, were developed. Dextromethorphan and dextrorphan hemisuccinates were linked to bovine serum albumin and specific antisera against each immunogen were raised in rabbits. The sensitivity of the ELISA was high (limit of detection 740 and 600 pg.ml-1 for dextromethorphan and dextrorphan, respectively). Intra- and interassay variation was less than 10%, and cross-reactivity between the two compounds was less than 1%. The ELISA was employed to phenotype 216 French subjects. The frequency of poor metabolizers was 5.1%.
ISSN:0031-6970
1432-1041
DOI:10.1007/BF00280940