A method for the hyaluronic acid synthetase assay in human skin

A method for the determination of hyaluronic acid synthetase activity of human skin is described. Skin samples were crush-homogenized, and incubated with UDP-14C-glucuronic acid and UDP-N-acetylglucosamine in Tris-HCl-buffer containing MgCl2. After papain digestion of the samples, the 14C-labeled hy...

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Bibliographic Details
Published in:Archives of Dermatological Research 1982-01, Vol.273 (3-4), p.199-204
Main Authors: Larjava, H, Vaarala, J, Saarni, H, Penttinen, R, Hopsu-Havu, V K
Format: Article
Language:English
Subjects:
Adrenal Cortex Hormones - therapeutic use
Adult
Carbon Radioisotopes
Chromatography, Gel
Female
Glucuronosyltransferase - analysis
Glycosyltransferases
Humans
Hyaluronan Synthases
Membrane Proteins
Psoriasis - drug therapy
Psoriasis - enzymology
Skin - enzymology
Transferases
Uridine Diphosphate Glucuronic Acid
Xenopus Proteins
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Summary:A method for the determination of hyaluronic acid synthetase activity of human skin is described. Skin samples were crush-homogenized, and incubated with UDP-14C-glucuronic acid and UDP-N-acetylglucosamine in Tris-HCl-buffer containing MgCl2. After papain digestion of the samples, the 14C-labeled hyaluronic acid was separated in Sephadex G 50 chromatography. The radioactivity incorporated into hyaluronic acid was an expression of the activity of hyaluronic acid synthetase. This activity was found to be increased in skin biopsies obtained from psoriatic lesions and decreased after local treatment with potent corticoids.
ISSN:0340-3696
1432-069X
DOI:10.1007/BF00409247