Ovarian Substance Induces Steroid Production in Cultured Granulosa Cells

The rat ovary produces an apparently low molecular weight substance that mimics the action of follitropin (FSH) on ovarian granulosa cells in culture. Similar to FSH action, the ovarian substance (OS) induces temporal cell rounding and, later on, intensive progestin production. However, unlike FSH,...

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Bibliographic Details
Published in:In Vitro 1982-12, Vol.18 (12), p.980-989
Main Authors: Orly, Joseph, Farkash, Yigal, Hershkovits, Nitzan, Mizrahi, Lina, Weinberger, Patricia
Format: Article
Language:English
Subjects:
20-alpha-Dihydroprogesterone - biosynthesis
Agonists
Animals
Cell culture techniques
Cells, Cultured
Cultured cells
Cyclic AMP - metabolism
Female
Follicle Stimulating Hormone - pharmacology
Granulosa cells
Granulosa Cells - drug effects
Granulosa Cells - physiology
Hormonal regulation
Hormones
Kinetics
Low molecular weights
Male
Ovaries
Ovary - physiology
Progesterone - analogs & derivatives
Rats
Rats, Inbred Strains
Steroidogenesis
Steroids
Symposium
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Summary:The rat ovary produces an apparently low molecular weight substance that mimics the action of follitropin (FSH) on ovarian granulosa cells in culture. Similar to FSH action, the ovarian substance (OS) induces temporal cell rounding and, later on, intensive progestin production. However, unlike FSH, OS does not induce accumulation of cyclic AMP (cAMP) in the granulosa cells. The ovarian factor cannot be cAMP as its action is not abolished by phosphodiesterase (PDE) treatment. Neither is it a possible PDE inhibitor, as it does not augment cAMP accumulation in granulosa cells or Friend erythroleukemic cells induced by FSH or PGE₁, respectively. The factor is still active after heating for 20 min at 90° C but is rapidly inactivated by alkali treatment. In addition, treatment with various proteases did not abolish the steroidogenic activity. These findings suggest a possible novel intraovarian regulator of the granulosa cell function.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02796372