Extracentromeric connections between sister chromatids demonstrated in human chromosomes induced to condense asymmetrically

Extracentromeric chromatin fibers were proposed to hold sister chromatids together in mitotic chromosomes examined by electron microscopy, but their existence in living cells has not been demonstrated yet. We have performed an in vitro BrdU-H33258 treatment which induced a differential rate of conde...

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Bibliographic Details
Published in:Human genetics 1982-01, Vol.62 (4), p.324-326
Main Authors: Goyanes, V J, Méndez, J
Format: Article
Language:English
Subjects:
Chromatids - ultrastructure
Chromatin - ultrastructure
Chromosome Banding
Chromosome Inversion
Chromosomes, Human - ultrastructure
Crossing Over, Genetic
Humans
Mitosis
Sister Chromatid Exchange
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Summary:Extracentromeric chromatin fibers were proposed to hold sister chromatids together in mitotic chromosomes examined by electron microscopy, but their existence in living cells has not been demonstrated yet. We have performed an in vitro BrdU-H33258 treatment which induced a differential rate of condensation to each sister chromatid, thus producing asymmetrically condensing chromosomes. The fast condensing chromatid pulled the slower sister one, both bending in parallel. Bent chromatids appeared reciprocally connected by loops of chromatin fibers, suggesting they were the links which permitted the physical interplay between the differently condensing chromatids. When sister chromatid exchanges (SCE) intercalated a fast-condensing fragment in the slow-condensing chromatid or vice versa, the chromosome inverted its curvature at the SCE-point.
ISSN:0340-6717
1432-1203
DOI:10.1007/bf00304548