A study of calcium compartments in rat brain cortex thin slices: effects of veratridine, lithium and of a mitochondrial uncoupler

The efflux kinetics of 45Ca from rat brain cortex thin slices previously equilibrated with it, was studied in a superfusion system. Two first order kinetic components of efflux from the tissue were found: k2 = 0.0667 min-1, that was unchanged by lowering the temperature from 37 degrees C to 15 degre...

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Bibliographic Details
Published in:Neurochemical research 1982-11, Vol.7 (11), p.1427-1435
Main Authors: Rubiales de Barioglio, S, Orrego, F
Format: Article
Language:English
Subjects:
Animals
Caffeine - pharmacology
Calcium - metabolism
Carbonyl Cyanide m-Chlorophenyl Hydrazone - pharmacology
Cell Compartmentation
Cerebral Cortex - drug effects
Cerebral Cortex - metabolism
Kinetics
Lithium - pharmacology
Nitriles - pharmacology
Rats
Rats, Inbred Strains
Veratridine - pharmacology
Veratrine - analogs & derivatives
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Summary:The efflux kinetics of 45Ca from rat brain cortex thin slices previously equilibrated with it, was studied in a superfusion system. Two first order kinetic components of efflux from the tissue were found: k2 = 0.0667 min-1, that was unchanged by lowering the temperature from 37 degrees C to 15 degrees, and k3 = 0.0167 min-1 at 37 degrees C, that was reduced to 0.0897 min-1 at 15 degrees C. This suggests that k2 represents efflux from the extracellular space, and k3 that from the cellular compartment. Addition of the mitochondrial uncoupler carbonyl cyanide, m-chlorophenylhydrazone (CCCP) (10(-5)M) increased the efflux fractional rate constant of 45Ca by 35%, while no change in efflux was induced by 10 mM caffeine. Veratridine (10(-5)M) drastically reduced 45Ca efflux if superfusion was with physiological salt solution (150 mM sodium present), but not if 50 mM lithium replaced an equivalent amount of sodium in the superfusion fluid. This lithium-containing solution did not affect 45Ca efflux in the absence of veratridine. These results indicate that mitochondria accumulate only a minor fraction of intracellular 45Ca; that 45Ca possibly turns over very rapidly in the endoplasmic reticulum, and that most of 45Ca is present in a different, non-mitochondrial, non endoplasmic reticular compartment, the nature of which can be only conjectured.
ISSN:0364-3190
1573-6903
DOI:10.1007/BF00966071