Chromatographic separation and photoelectrochemical detection of sodium nitroprusside and its degradative and metabolic products

Ion-pair chromatography has been used for the separation of nitroprusside ion and its photochemical hydrolytic and metabolic products. Organic modifier and pH were adjusted for maximum separation of the ions. Methanol was selected as the organic modifier in a pH range 5–8 and tetrabutylammonium perc...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1990, Vol.8 (6), p.521-526
Main Authors: Ayers, Tracy H., Metwally, Mohammed E.-S., Honigberg, Irwin L.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Chromatography
degradation ions
Electrochemistry
Ferricyanides - analysis
Ferricyanides - isolation & purification
Ferricyanides - metabolism
General pharmacology
Indicators and Reagents
ion-pair chromatography
Medical sciences
Nitroprusside
Nitroprusside - analysis
Nitroprusside - isolation & purification
Nitroprusside - metabolism
Pharmacology. Drug treatments
Photochemistry
photoelectrochemical detector
Solvents
thiocyanate
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Summary:Ion-pair chromatography has been used for the separation of nitroprusside ion and its photochemical hydrolytic and metabolic products. Organic modifier and pH were adjusted for maximum separation of the ions. Methanol was selected as the organic modifier in a pH range 5–8 and tetrabutylammonium perchlorate was used as the ion-pairing reagent. Ions were detected with a photoelectrochemical detector as described by Krull. A modification of this procedure was used to detect nitroprusside ion in spiked serum samples.
ISSN:0731-7085
1873-264X
DOI:10.1016/0731-7085(90)80062-T