Induction of a heat shock gene (hsp70) in rabbit retinal ganglion cells detected by in situ hybridization with plastic-embedded tissue

Elevation of body temperature by 2-3 degrees C induces a 2.7 kilobase hsp70 mRNA species in the rabbit retina within 1 hr. In situ hybridization with thin sections derived from plastic-embedded tissue permitted a higher level of resolution of retinal cell types compared to procedures which involved...

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Bibliographic Details
Published in:Neurochemical research 1990-12, Vol.15 (12), p.1229-1235
Main Authors: MASING, T. E, RUSH, S. J, BROWN, I. R
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Heat-Shock Proteins - genetics
Histological Techniques
Hot Temperature
Nucleic Acid Hybridization
Olfactory system and olfaction. Gustatory system and gustation
Plastics
Rabbits
Retina - cytology
Retinal Ganglion Cells - cytology
Retinal Ganglion Cells - physiology
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Vertebrates: nervous system and sense organs
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Summary:Elevation of body temperature by 2-3 degrees C induces a 2.7 kilobase hsp70 mRNA species in the rabbit retina within 1 hr. In situ hybridization with thin sections derived from plastic-embedded tissue permitted a higher level of resolution of retinal cell types compared to procedures which involved the use of frozen tissue sections. A prominent induction of hsp70 mRNA in retinal ganglion cells was observed when an hsp70 riboprobe was utilized for in situ hybridization. These results indicate that this neuronal cell type responds rapidly to fever-like body temperatures by inducing one of the major heat shock genes.
ISSN:0364-3190
1573-6903
DOI:10.1007/BF01208584