Cell Culture Quality Control by Rapid Isoenzymatic Characterization

Procedures that involve cell cultures require careful quality control to avoid inter- and intraspecies contamination. We have developed an electrophoresis technique that can be used routinely in cell culture laboratories to monitor cell line integrity. The method involves the isoenzymatic separation...

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Bibliographic Details
Published in:In Vitro 1983-01, Vol.19 (1), p.16-24
Main Authors: Halton, D M, Peterson, Jr, W D, Hukku, B
Format: Article
Language:English
Subjects:
Animals
B lymphocytes
Cats
Cell culture techniques
Cell Line
Cell lines
Chickens
Cultured cells
Cytological Techniques
Dehydrogenases
Electrophoresis, Agar Gel
Enzymes
Haplorhini
Humans
Isoenzymes - genetics
Isoenzymes - isolation & purification
Mice
Phenotype
Phenotypes
Quality assurance
Quality Control
Rabbits
Rats
Tumor cell line
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Summary:Procedures that involve cell cultures require careful quality control to avoid inter- and intraspecies contamination. We have developed an electrophoresis technique that can be used routinely in cell culture laboratories to monitor cell line integrity. The method involves the isoenzymatic separation of nine polymorphic enzymes, three of which can be used for cell line species determinations and seven of which can be used for human cell line characterizations. Examples of how the system has been applied to both inter- and intraspecies identifications are described. The routine application of this protocol would be a valuable asset for laboratories concerned with establishing effective cell culture quality control.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02617989