Whole animal cell sorting of Drosophila embryos

Use of primary culture cells has been limited by the inability to purify most types of cells, particularly cells from early developmental stages. In whole animal cell sorting (WACS), live cells derived from animals harboring a lacZ transgene arc purified according to their level of beta-galactosidas...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1991-01, Vol.251 (4989), p.81-85
Main Authors: Krasnow, M.A. (Stanford University, Stanford, CA), Cumberledge, S, Manning, G, Herzenberg, L.A, Nolan, G.P
Format: Article
Language:English
Subjects:
Animal cells
Animal embryology
Animals
Animals, Genetically Modified
Cell culture
Cell lines
Cell Separation - methods
Cells, Cultured
Cellular biology
Cellular differentiation
CULTIVO DE CELULAS
CULTURE DE CELLULE
Cultured cells
Cultures and culture media
Cytology
DESARROLLO EMBRIONARIO
DEVELOPPEMENT EMBRYONNAIRE
Disaccharides
Drosophila
DROSOPHILA MELANOGASTER
Drosophila melanogaster - embryology
Embryology
Embryonic cells
Embryos
Escherichia coli - genetics
EXPRESION GENICA
EXPRESSION DES GENES
Flow Cytometry
Fluorescence
Fluorescent Dyes
Galactosides - analysis
Genetics
Lac Operon
Methods
Neural stem cells
Neurons
Neurons - cytology
Stem cells
Stem Cells - cytology
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Summary:Use of primary culture cells has been limited by the inability to purify most types of cells, particularly cells from early developmental stages. In whole animal cell sorting (WACS), live cells derived from animals harboring a lacZ transgene arc purified according to their level of beta-galactosidase expression with a fluorogenic beta-galactosidase substrate and fluorescence-activated cell sorting. With WACS, incipient posterior compartment cells that express the engrailed gene were purified from early Drosophila embryos. Neuronal precursor cells were also purified, and they differentiated into neurons with high efficiency in culture. Because there are many lacZ strains, it may be possible to purify most types of Drosophila cells. The same approach is also applicable to other organisms for which germ-line transformation is possible
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1898782