Characterization of Borrelia burgdorferi associated antigens by monoclonal antibodies

In this paper, we present a series of murine mAb recognizing B. burgdorferi antigens. The antibodies were characterized by immuno-blotting and immuno-fluorescence studies using isolates of B. burgdorferi from North America and Europe, respectively. Moreover, reactivity of the antibodies with recombi...

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Bibliographic Details
Published in:Immunobiology (1979) 1990-11, Vol.181 (4-5), p.357-366
Main Authors: KRAMER, M. D, SCHAIBLE, U. E, WALLICH, R, MOTER, S. E, PETZOLDT, D, DIMON, M. M
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial
Antibodies, immunoglobulins
Antibodies, Monoclonal
Antibody Specificity
Antigens, Bacterial
Bacterial Proteins - immunology
Biological and medical sciences
Borrelia burgdorferi Group - immunology
Borrelia burgdorferi Group - isolation & purification
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Mice
Molecular immunology
Monoclonal antibodies
Recombinant Proteins - immunology
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Summary:In this paper, we present a series of murine mAb recognizing B. burgdorferi antigens. The antibodies were characterized by immuno-blotting and immuno-fluorescence studies using isolates of B. burgdorferi from North America and Europe, respectively. Moreover, reactivity of the antibodies with recombinant B. burgdorferi flagellin and OspA was studied. The results suggest these anti-B. burgdorferi mAb as valuable tools for the serological analysis of B. burgdorferi isolates and for affinity-purification of the respective proteins. Moreover, these mAb appear suitable to classify antigenic variants of B. burgdorferi and to study the protective capacity of antibodies in a murine model for B. burgdorferi infection.
ISSN:0171-2985
1878-3279
DOI:10.1016/S0171-2985(11)80504-8