Characterization and Expression of the Antigen Present on Resident Rat Macrophages Recognized by Monoclonal Antibody ED2

Because of the absence of a specific marker for labeling resident macrophages in the rat, there is almost no information available regarding the properties of individual resident macrophages in different organs. The recently described and in our laboratory developed mAb ED2, has been shown to exclus...

Full description

Saved in:
Bibliographic Details
Published in:Immunobiology (1979) 1990-12, Vol.182 (1), p.88-99
Main Authors: Barbé, Ellis, Damoiseaux, Jan G.M.C., Döpp, Ed A., Dijkstra, Christine D.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Antigens, Differentiation - analysis
Antigens, Differentiation - immunology
Antigens, Surface
Autoradiography
Biological and medical sciences
Bone Marrow Cells
Cell Adhesion
Cell Differentiation
Cells, Cultured
Culture Media
Electrophoresis, Polyacrylamide Gel
Extracellular Matrix Proteins - pharmacology
Fibronectins - pharmacology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunobiology
Immunohistochemistry
Macrophages - cytology
Macrophages - immunology
Monocytes, macrophages
Myeloid cells: ontogeny, maturation, markers, receptors
Precipitin Tests
Rats
Rats, Inbred Strains
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Because of the absence of a specific marker for labeling resident macrophages in the rat, there is almost no information available regarding the properties of individual resident macrophages in different organs. The recently described and in our laboratory developed mAb ED2, has been shown to exclusively recognize resident macrophages. The present study examines expression, function and structure of the ED2 antigen to obtain more information about the marker and therefore, more information about resident macrophages. In earlier studies, the expression of ED2 could not be induced by a range of macrophage stimulating factors under non-adherent culture conditions. We show a highly inducible expression of the ED2 antigen under adhering, non proliferating conditions as well as in long-term bone marrow cultures. ED2 appears to recognize a surface protein on resident macrophages consisting of three protein chains of 175, 160, and 95 kDa.
ISSN:0171-2985
1878-3279
DOI:10.1016/S0171-2985(11)80586-3