Characterization of lymphoid cells by two-dimensional mini gel electrophoresis of proteins

Cytosol proteins from normal lymphocytes, leukemic lymphocytes, and different cultured lymphoid cell lines were separated by two-dimensional mini gel electrophoresis. By staining with Coomassie blue, specific protein patterns were obtained. Very similar gel maps were produced by the cytosol proteins...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cancer research and clinical oncology 1983-01, Vol.105 (2), p.166-172
Main Authors: Hirsch, F W, Bröckl, C, Bross, K J, Dölken, G
Format: Article
Language:English
Subjects:
Adult
Aged
Blood Proteins - analysis
Cell Line
Cytosol - analysis
Electrophoresis - methods
Female
Granulocytes - analysis
Humans
Leukemia, Lymphoid - blood
Leukemia, Myeloid - blood
Leukocytes - analysis
Lymphocytes - analysis
Male
Middle Aged
Monocytes - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cytosol proteins from normal lymphocytes, leukemic lymphocytes, and different cultured lymphoid cell lines were separated by two-dimensional mini gel electrophoresis. By staining with Coomassie blue, specific protein patterns were obtained. Very similar gel maps were produced by the cytosol proteins of chronic lymphocytic leukemia cells, hairy cells, and of in vitro grown B cells. Protein 36/6.2 (molecular weight/isoelectric point), consistently present in these cells, could not be demonstrated in normal lymphocytes. For the comparison of control Raji cells--an Epstein-Barr-Virus (EBV)-DNA carrying Burkitt's lymphoma cell line--with Raji cells induced for early antigen (EA) production, 35S-methionine-labelled total cell lysates were analyzed. In the induced cells, an additional protein (100/5.5) was found; this might be one of the immunologically defined EBV-associated antigens. These results demonstrate that two-dimensional mini gel electrophoresis can be useful for the characterization of leukemic cells in addition to the morphological, cytochemical, and surface marker analyses.
ISSN:0171-5216
1432-1335
DOI:10.1007/BF00406928