Collagen Formation by the Hepatocyte in Primary Monolayer Culture and in vivo

Immunohistochemical techniques were used to confirm biochemical evidence that parenchymal cells isolated from adult rat liver and maintained in nonreplicating monolayer culture for 2 days synthesized type IV basement membrane collagen. On continued incubation in serum-free medium, the hepatocytes al...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1983-03, Vol.219 (4590), p.1343-1345
Main Authors: Diegelmann, Robert F., Guzelian, Philip S., Gay, Renate, Gay, Steffen
Format: Article
Language:English
Subjects:
Albumins
Animals
Antibodies
Basement membrane
Basement Membrane - metabolism
Cells, Cultured
Collagen
Collagen - biosynthesis
Collagen - immunology
Collagens
Cultured cells
Epithelial cells
Gels
Hepatocytes
Liver
Liver - cytology
Liver - metabolism
Liver diseases
Molecular Weight
Pathology
Rats
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Summary:Immunohistochemical techniques were used to confirm biochemical evidence that parenchymal cells isolated from adult rat liver and maintained in nonreplicating monolayer culture for 2 days synthesized type IV basement membrane collagen. On continued incubation in serum-free medium, the hepatocytes also synthesized the interstitial collagens, types I and III. Consistent with these results in culture, type IV collagen was localized to the hepatocytes in slices of pathologic rat liver. Hence collagen formation is a previously unrecognized function of the hepatocyte that may be important in the pathogenesis of liver fibrosis or cirrhosis.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.6828863