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Collagen Formation by the Hepatocyte in Primary Monolayer Culture and in vivo

Immunohistochemical techniques were used to confirm biochemical evidence that parenchymal cells isolated from adult rat liver and maintained in nonreplicating monolayer culture for 2 days synthesized type IV basement membrane collagen. On continued incubation in serum-free medium, the hepatocytes al...

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Published in:	Science (American Association for the Advancement of Science) 1983-03, Vol.219 (4590), p.1343-1345
Main Authors:	Diegelmann, Robert F., Guzelian, Philip S., Gay, Renate, Gay, Steffen
Format:	Article
Language:	English
Subjects:	Albumins Animals Antibodies Basement membrane Basement Membrane - metabolism Cells, Cultured Collagen Collagen - biosynthesis Collagen - immunology Collagens Cultured cells Epithelial cells Gels Hepatocytes Liver Liver - cytology Liver - metabolism Liver diseases Molecular Weight Pathology Rats
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description	Immunohistochemical techniques were used to confirm biochemical evidence that parenchymal cells isolated from adult rat liver and maintained in nonreplicating monolayer culture for 2 days synthesized type IV basement membrane collagen. On continued incubation in serum-free medium, the hepatocytes also synthesized the interstitial collagens, types I and III. Consistent with these results in culture, type IV collagen was localized to the hepatocytes in slices of pathologic rat liver. Hence collagen formation is a previously unrecognized function of the hepatocyte that may be important in the pathogenesis of liver fibrosis or cirrhosis.
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subjects	Albumins Animals Antibodies Basement membrane Basement Membrane - metabolism Cells, Cultured Collagen Collagen - biosynthesis Collagen - immunology Collagens Cultured cells Epithelial cells Gels Hepatocytes Liver Liver - cytology Liver - metabolism Liver diseases Molecular Weight Pathology Rats
title	Collagen Formation by the Hepatocyte in Primary Monolayer Culture and in vivo
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