Rapid and efficient detection of genetic polymorphism in wheat through amplification by polymerase chain reaction

The polymerase chain reaction (PCR) was used to amplify genomic DNA of several wheat genotypes. The oligonucleotides used as primers were the terminal sequences of a gamma-gliadin gene. The electrophoretic analysis of the PCR products showed specific bands which revealed both inter- and intra-specif...

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Bibliographic Details
Published in:Plant molecular biology 1990-07, Vol.15 (1), p.169-171
Main Authors: D'Ovidio, R. (Universita della Tuscia, Viterbo (Italy). Dipartimento di Agrobiologia e Agrochimica), Tanzarella, O.A, Porceddu, E
Format: Article
Language:English
Subjects:
ADN
Agronomy. Soil science and plant productions
Base Sequence
Biological and medical sciences
Classical and quantitative genetics
Classical and quantitative genetics. Population genetics. Molecular genetics
DNA
DNA - genetics
ELECTROFORESIS
ELECTROPHORESE
ELECTROPHORESIS
Fundamental and applied biological sciences. Psychology
Gene Amplification
Generalities. Genetics. Plant material
Genetic Markers
GENETIC POLYMORPHISM
Genetics and breeding of economic plants
GENOMAS
GENOME
GENOMES
GENOTIPOS
GENOTYPE
GENOTYPES
GRAINE
METHODE
METHODS
METODOS
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
Plants - genetics
POLIMORFISMO GENETICO
Polymerase Chain Reaction
Polymorphism, Genetic
POLYMORPHISME GENETIQUE
PROTEINAS
PROTEINE
PROTEINS
SECUENCIA NUCLEICA
SEEDS
SEMILLA
SEQUENCE NUCLEIQUE
TRITICUM
Triticum - genetics
Triticum aestivum
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Summary:The polymerase chain reaction (PCR) was used to amplify genomic DNA of several wheat genotypes. The oligonucleotides used as primers were the terminal sequences of a gamma-gliadin gene. The electrophoretic analysis of the PCR products showed specific bands which revealed both inter- and intra-specific genetic polymorphism among the examined genotypes. The technique is proposed as a very simple and efficient alternative to RFLP markers.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00017737