Primed Lymphocyte Testing Specificity of Alloreactive Lymphocyte Clones for HLA-B Locus Determinants

We have generated two alloreactive T-lymphocyte clones that recognize the products of the HLA-B locus. The primed lymphocyte testing (PLT) reactivity of clone HJ21 was monospecific for the HLA-B antigen Bw49, a subtype specificity of HLA-Bw21. The PLT specificity of clone RD3 was strongly associated...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1983-03, Vol.80 (5), p.1440-1444
Main Authors: Zeevi, Adriana, Scheffel, Christi, Annen, Kathleen, Duquesnoy, Rene J.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - immunology
Antigens
Cloning, Molecular
Cultured cells
Epitopes - immunology
HLA A antigens
HLA antigens
HLA Antigens - immunology
HLA B antigens
HLA D antigens
Humans
Lymphocyte Activation
Lymphocytes
Molecules
T lymphocytes
T-Lymphocytes - immunology
Tissue culture techniques
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Summary:We have generated two alloreactive T-lymphocyte clones that recognize the products of the HLA-B locus. The primed lymphocyte testing (PLT) reactivity of clone HJ21 was monospecific for the HLA-B antigen Bw49, a subtype specificity of HLA-Bw21. The PLT specificity of clone RD3 was strongly associated with HLA-Bw21. Several monoclonal antibodies against class I HLA molecules inhibited the PLT responses of these clones whereas class II monoclonal antibodies had no effect. These observations provide experimental evidence that class I products of the HLA-B locus may directly be involved in lymphocyte alloactivation.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.80.5.1440