Characterization of a New Human Endometrial Carcinoma (RL95-2) Established in Tissue Culture

A new human endometrial cell line, RL95-2, derived from a Grade 2 moderately differentiated adenosquamous carcinoma of the endometrium has been passaged successfully in cell culture for more than 2 yr. The cells are characteristically epithelioid with well-defined junctional complexes, tonofilaments...

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Bibliographic Details
Published in:In Vitro 1983-03, Vol.19 (3), p.147-158
Main Authors: Dennis L. Way, David S. Grosso, Davis, John R., Earl A. Surwit, C. D. Christian
Format: Article
Language:English
Subjects:
Adenocarcinoma - pathology
Adenocarcinoma - ultrastructure
Aged
Carcinoma, Squamous Cell - pathology
Carcinoma, Squamous Cell - ultrastructure
Cell culture techniques
Cell growth
Cell Line
Cell lines
Culture Techniques
Cultured cells
Cytoplasm - metabolism
Diploidy
Endometrial neoplasms
Epithelial cells
Epithelioid cells
Estrogen receptors
Female
Fluorescent Antibody Technique
Humans
Karyotyping
Microscopy, Electron, Scanning
Receptors, Estrogen - analysis
Tumor cell line
Uterine Neoplasms - metabolism
Uterine Neoplasms - pathology
Uterine Neoplasms - ultrastructure
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Summary:A new human endometrial cell line, RL95-2, derived from a Grade 2 moderately differentiated adenosquamous carcinoma of the endometrium has been passaged successfully in cell culture for more than 2 yr. The cells are characteristically epithelioid with well-defined junctional complexes, tonofilaments, filopodialike extensions, and surface microvilli. Nuclei are large, irregular, and invaginated frequently with multiple, prominent, lamellar nucleoli. The cells have a log phase doubling time of 22 to 34 h followed by continued growth at a reduced rate with no apparent plateau phase. They exhibit a strong tendency for piling up as well as for the formation of glandlike dome structures. Karyotypically the line is trisomic 8 (47,XX,+8) and has an 8% frequency of polyploidization. Both cytoplasmic and nuclear estrogen receptors are present. Antihuman α-keratin characterizes the cell line as epithelial, nonstromal. The RL95-2 cell line may provide a useful in vitro system for the investigation of the endocrine regulation of endometrial neoplasia.
ISSN:0073-5655
1475-2689
DOI:10.1007/bf02618053