Biosynthesis of O-phosphoserine-containing phosphoproteins by isolated bone cells of mouse calvaria

Five groups of isolated bone cells from mouse calvaria were incubated with [ 3H]serine and the presence and amount of O[ 3H]phosphoserine used as an indication of phosphoprotein synthesis. Cells in the osteoblastic fraction were the most active in synthesizing phosphoproteins, and unlike the other c...

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Bibliographic Details
Published in:FEBS letters 1983-04, Vol.154 (1), p.116-120
Main Authors: Gotoh, Y., Sakamoto, M., Sakamoto, S., Glimcher, M.J.
Format: Article
Language:English
Subjects:
Animals
Bone and Bones - metabolism
Bone cell
Chromatography, Ion Exchange
Gdn-HCl
guanidine hydrochloride
In Vitro Techniques
MEM
Mice
minimum essential medium (Eagle)
Mouse calvaria
O-phosphoserine
parathyroid hormone
Phosphoprotein synthesis
Phosphoproteins - biosynthesis
Phosphoserine
Phosphoserine - biosynthesis
PTH
Ser(P)
Serine - analogs & derivatives
Skull
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Summary:Five groups of isolated bone cells from mouse calvaria were incubated with [ 3H]serine and the presence and amount of O[ 3H]phosphoserine used as an indication of phosphoprotein synthesis. Cells in the osteoblastic fraction were the most active in synthesizing phosphoproteins, and unlike the other cell groups, released most of the phosphoproteins into the tissue culture medium. When subjected to molecular sieving and ion-exchange chromatography, the phosphoproteins synthesized by the bone cells of the osteoblastic group behaved like the phosphoproteins extracted from mouse calvaria by EDTA.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(83)80886-2