Absorption of horse-radish peroxidase by the conjunctival epithelium of monkeys and rabbits

Horse-radish peroxidase was instilled into the conjunctival sac of rabbits and Cynomolgus monkeys. After an interval of 5, 30 or 60 min the conjunctival epithelium was studied by electron microscopy. The tracer was found to be absorbed predominantly by type-V cells, which are rich in mitochondria; t...

Full description

Saved in:
Bibliographic Details
Published in:Graefe's archive for clinical and experimental ophthalmology 1983, Vol.220 (1), p.13-18
Main Authors: Steuhl, P, Rohen, J W
Format: Article
Language:English
Subjects:
Absorption
Animals
Conjunctiva - metabolism
Conjunctiva - ultrastructure
Epithelium - metabolism
Epithelium - ultrastructure
Horseradish Peroxidase - metabolism
Macaca fascicularis
Microscopy, Electron
Peroxidases - metabolism
Rabbits
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Horse-radish peroxidase was instilled into the conjunctival sac of rabbits and Cynomolgus monkeys. After an interval of 5, 30 or 60 min the conjunctival epithelium was studied by electron microscopy. The tracer was found to be absorbed predominantly by type-V cells, which are rich in mitochondria; this process was found to occur more rapidly in the rabbit than in the monkey. The particles were primarily incorporated into pinocytotic vesicles and phagosomes and were then either digested by phagolysosomes or transported through the basal portion of the surface epithelial cells into the expanding intercellular spaces distal to the junctional complexes.
ISSN:0721-832X
1435-702X
DOI:10.1007/BF02307010