Use of an Enzyme-Linked Immunosorbent Assay Technique in the Differential Diagnosis of Active Pulmonary Tuberculosis in Humans

Sera from patients with active pulmonary tuberculosis and pulmonary diseases frequently mimicking tuberculosis were assayed for immunoglobulin G antibody activity to purified protein derivative (PPD) by an enzyme-linked immunosorbent assay. A method of standardization was developed to limit assay va...

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Bibliographic Details
Published in:The Journal of infectious diseases 1983-03, Vol.147 (3), p.523-530
Main Authors: Kalish, S. B., Radin, R. C., Phair, J. P., Levitz, D., Zeiss, C. R., Metzger, E.
Format: Article
Language:English
Subjects:
Antibodies
Antibody Formation
Antigens
Antigens, Bacterial - immunology
BCG Vaccine - immunology
Diagnosis, Differential
Enzyme linked immunosorbent assay
Humans
Immunoenzyme Techniques
Immunoglobulin G - immunology
Infections
Lung Diseases - diagnosis
Microbiology and Laboratory Diagnosis
Mycobacterium tuberculosis
Mycobacterium tuberculosis - immunology
Pulmonary tuberculosis
Sarcoidosis
Skin Tests
Tuberculin
Tuberculosis vaccine
Tuberculosis, Pulmonary - diagnosis
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Summary:Sera from patients with active pulmonary tuberculosis and pulmonary diseases frequently mimicking tuberculosis were assayed for immunoglobulin G antibody activity to purified protein derivative (PPD) by an enzyme-linked immunosorbent assay. A method of standardization was developed to limit assay variation. Patients with active pulmonary tuberculosis had a significantly greater mean level of antibody than had patients with atypical tuberculosis (P = 0.005), sarcoidosis (P = 0.0001), histoplasmosis (P = 0.004), blastomycosis (P = 0.008), or cryptococcosis (P = 0.017), patients who had received bacille Calmette-Guerin vaccination (P = 0.003) or who had a history of treated tuberculosis (P = 0.003), and PPD skin test-positive and skin test-negative control subjects (P = 0.001). This technique may have potential use as a rapid diagnostic aid in evaluating patients with suspected active pulmonary tuberculosis.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/147.3.523