Purification of Highly Radioactive Mouse Interferon Produced by Ehrlich Ascites Tumour Cells Induced by Sendai Virus

1 Institut National de la Santé et de la Recherche Médicale, U. 43, Hôpital Saint Vincent-de-Paul, 74 Avenue Denfert-Rochereau, 75014 Paris and 2 Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France Mouse interferon (IFN) was produced to high titres after inductio...

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Bibliographic Details
Published in:Journal of general virology 1983-02, Vol.64 (2), p.285-290
Main Authors: Dussaix, E, Gregoire, A, Chany, C, Thang, D. C, Thang, M. N
Format: Article
Language:English
Subjects:
Animals
Carcinoma, Ehrlich Tumor
Cells, Cultured
Chromatography, Agarose
Electrophoresis, Polyacrylamide Gel
Interferons - biosynthesis
Interferons - isolation & purification
Mice
Parainfluenza Virus 1, Human - immunology
Sendai virus
Tritium
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Summary:1 Institut National de la Santé et de la Recherche Médicale, U. 43, Hôpital Saint Vincent-de-Paul, 74 Avenue Denfert-Rochereau, 75014 Paris and 2 Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France Mouse interferon (IFN) was produced to high titres after induction of Ehrlich ascites tumour cells with Sendai virus by using an improved procedure. The IFN molecules were labelled during their synthesis by the incorporation of [ 3 H]leucine and [ 3 H]lysine. Electrophoretically homogeneous labelled IFN with a molecular weight of 34000 was obtained after a two-step purification procedure using poly(I)-agarose and octyl-Sepharose column chromatography followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The specific radioactivity of this IFN was about 10 ct/min/IFN unit. Keywords: interferon (MuIFN), purification, internal labelling Received 29 April 1982; accepted 30 August 1982.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-64-2-285