Heterogeneity in the Genome RNAs and Polypeptides of Five Members of a Novel Group of Rotavirus-like Viruses Isolated from Aquatic Animals

• Published in: Journal of general virology 1991-01, Vol.72 (1), p.181-184
• Main Authors: Samal, Siba K, Dopazo, Carlos P, Subramanian, Kothandaraman, Lupiani, Blanca, Mohanty, Sashi B, Hetrick, Frank M
• Format: Article
• Language: English
• Subjects: Animals; Bass - microbiology; Biological and medical sciences; Brackish; Electrophoresis, Agar Gel; Electrophoresis, Polyacrylamide Gel; Fishes - microbiology; Flatfishes - microbiology; Freshwater; Fundamental and applied biological sciences. Psychology; Genes, Viral; Genetics; Marine; Microbiology; Morone saxatilis; Nucleic Acid Hybridization; Osmerus mordax; RNA, Double-Stranded - genetics; RNA, Double-Stranded - isolation & purification; RNA, Viral - genetics; RNA, Viral - isolation & purification; Rotavirus - genetics; Rotavirus - isolation & purification; Salmon - microbiology; Scophthalmus maximus; Viral Proteins - genetics; Viral Proteins - isolation & purification; Virion - genetics; Virion - isolation & purification; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-72-1-181

1 College of Veterinary Medicine and 2 Microbiology Department, University of Maryland, College Park, Maryland 20742, U.S.A. Biochemical characteristics of five rotavirus-like viruses isolated from striped bass ( Morone saxatilis ), turbot ( Scophthalmus maximus ), smelt ( Osmerus mordax ) and Atlantic salmon ( Salmo salar ) in North America and Europe were compared. The genome of each isolate was composed of 11 segments of dsRNA and each isolate had a unique electropherotype in polyacrylamide gels. Agarose gel electrophoresis showed similar RNA profiles for all four isolates from North America, whereas the RNA profile of the isolate from Europe was different. Analysis of virion proteins revealed that each virus had five structural proteins ranging in M r from 130000 to 34000. Each isolate had a unique polypeptide profile but their overall polypeptide patterns were similar. Reciprocal RNA-RNA blot hybridization demonstrated that all these rotavirus-like viruses cross-hybridized with each other except for the isolate from Europe which did not hybridize with the RNA from any of the other isolates. No genetic relationship was found between these rotavirus-like viruses of fish and a true group A rotavirus (SA11). Received 3 May 1990; accepted 4 October 1990.