A secreted phosphoprotein marker for neoplastic transformation of both epithelial and fibroblastic cells

A wide variety of virally and spontaneously transformed fibroblasts secrete a major transformation-related phosphoprotein with a molecular weight (MW), depending on the species of origin, of about 62,000 (62K) 1–13 . Markedly elevated extracellular levels of this major 32 P-labelled protein are not...

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Bibliographic Details
Published in:Nature (London) 1983-04, Vol.302 (5910), p.714-715
Main Authors: Senger, Donald R., Asch, Bonnie B., Smith, Barbara D., Perruzzi, Carole A., Dvorak, Harold F.
Format: Article
Language:English
Subjects:
Acetoxyacetylaminofluorene
Animals
Cell Line
Cell Transformation, Neoplastic
Epithelium - physiology
Fibroblasts - physiology
Humanities and Social Sciences
letter
Liver
Molecular Weight
multidisciplinary
Phosphoproteins - isolation & purification
Phosphoproteins - metabolism
Rats
Rats, Inbred Strains
Science
Science (multidisciplinary)
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Summary:A wide variety of virally and spontaneously transformed fibroblasts secrete a major transformation-related phosphoprotein with a molecular weight (MW), depending on the species of origin, of about 62,000 (62K) 1–13 . Markedly elevated extracellular levels of this major 32 P-labelled protein are not simply linked to exponential growth 1 but instead are associated directly with transformation. The phosphoprotein is not antigenically related to p60 src , p60 c- src or simian virus 40 (SV40) non-viral T antigen 2 , and it is further distinguishable from SV40 non-viral T antigen (pp 53) on the basis of its electrophoretic mobility. In this study we have compared a variety of normal and transformed epithelial cells for secretion of this 32 P-labelled protein and have found that this marker distinguishes neoplastic from preneoplastic and normal mouse mammary epithelium and also identifies highly tumorigenic cells derived from guinea pig bile duct epithelium and rat liver epithelium. Because the classical phenotypic properties commonly associated with transformation of fibroblasts cannot be generally used to discriminate tumorigenic from non-tumorigenic epithelial cells 4–9 , this phosphoprotein, which identifies tumorigenic cells of both fibroblastic and epithelial origin, is likely to be of particular importance.
ISSN:0028-0836
1476-4687
DOI:10.1038/302714a0