Avidin binding of biotinylated corticotropins

The authors studied the effect on avidin binding of attaching peptide hormones to biotin and biotin analogues. It is concluded that the attachment of ACTH sub(1-24) to biocytin amide and its analogues exerts little influence on the affinity for avidin or succinoylavidin, a result that differs marked...

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Published in:Biochemistry (Easton) 1983-02, Vol.22 (4), p.904-909
Main Authors: Romovacek, Hana, Finn, Frances M, Hofmann, Klaus
Format: Article
Language:English
Subjects:
Adrenocorticotropic Hormone - analogs & derivatives
Adrenocorticotropic Hormone - chemical synthesis
Adrenocorticotropic Hormone - metabolism
Amino Acid Sequence
avidin
Avidin - metabolism
biotin
Chromatography, High Pressure Liquid
corticotropin
Indicators and Reagents
insulin
Ovalbumin - analogs & derivatives
Protein Binding
Structure-Activity Relationship
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container_title Biochemistry (Easton)
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creator Romovacek, Hana
Finn, Frances M
Hofmann, Klaus
description The authors studied the effect on avidin binding of attaching peptide hormones to biotin and biotin analogues. It is concluded that the attachment of ACTH sub(1-24) to biocytin amide and its analogues exerts little influence on the affinity for avidin or succinoylavidin, a result that differs markedly from that obtained with biotinylinsulin-succinoylavidin complexes. The attachment of insulin to biotin weakens significantly the avidin-biotin interaction presumably because the insulin molecular with its stable conformation exerts a steric impediment. Such an effect is not observed with the smaller, flexible biotinylated ACTH sub(1-24) molecules.
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It is concluded that the attachment of ACTH sub(1-24) to biocytin amide and its analogues exerts little influence on the affinity for avidin or succinoylavidin, a result that differs markedly from that obtained with biotinylinsulin-succinoylavidin complexes. The attachment of insulin to biotin weakens significantly the avidin-biotin interaction presumably because the insulin molecular with its stable conformation exerts a steric impediment. 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source ACS CRKN Legacy Archives
subjects Adrenocorticotropic Hormone - analogs & derivatives
Adrenocorticotropic Hormone - chemical synthesis
Adrenocorticotropic Hormone - metabolism
Amino Acid Sequence
avidin
Avidin - metabolism
biotin
Chromatography, High Pressure Liquid
corticotropin
Indicators and Reagents
insulin
Ovalbumin - analogs & derivatives
Protein Binding
Structure-Activity Relationship
title Avidin binding of biotinylated corticotropins
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