Relationship between a subunit of NADH dehydrogenase (complex I) and a protein family including subunits of cytochrome reductase and processing protease of mitochondria

• Published in: FEBS letters 1991-01, Vol.278 (1), p.75-78
• Main Authors: Ro¨hlen, D.A., Hoffmann, J., van der Pas, J.C., Nehls, U., Preis, D., Sackmann, U., Weiss, H.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Cytochrome Reductases - genetics; DNA - genetics; Endopeptidases - genetics; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; genes; Introns; Mitochondria; Mitochondria - enzymology; Molecular Sequence Data; NADH Dehydrogenase - genetics; NADH:ubiquinone redectase; Neurospora crassa; Oxidoreductases; Processing protease; Restriction Mapping; Sequence Homology, Nucleic Acid; Ubiquinol:cytochrome c reductase
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(91)80087-J

The primanry structure of a 40 kDa subunit of the respiratory chain NADH:ubiquinone reductase from Neurospora crassa was determined by sequencing cDNA, genomic DNA and the N-terminus of the mature protein. The gene which is interrupted by 7 introns encodes a preprotein consisting of 375 amino acids with a 26 amino acid long presequence typical for a mitochondrial targetting signal. The sequence of the mature subunit shows conspicuous similarities to the recently [(1989) Nature 339, 147–149] discovered protein family which includes subunits I and II of the ubiquinol:cytochrome c reductase, and the processing proteins, matrix processing peptidase and processing enhancing protein, of mitochondria. The possible role of the subunit is discussed.