The β globin 3' enhancer element confers regulated expression on the human γ globin gene in the human embryonic-fetal erythroleukemia cell line K562

We have constructed fusion genes comprised of gamma and beta globin elements and globin sequences linked to neomycin resistance (neoR) genes to define the cis acting sequences responsible for developmental stage-specific expression and induction of fetal globin genes in embryonic-fetal erythroleukem...

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Published in:Blood 1991-02, Vol.77 (4), p.855-860
Main Authors: DONOVAN-PELUSO, M, ACUTO, S, O'NEILL, D, HOM, A, MAGGIO, A, BANK, A
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cloning, Molecular
DNA, Recombinant
Embryo, Mammalian
Enhancer Elements, Genetic
Fetus - metabolism
Gene Expression Regulation
Genes
Globins - genetics
Hematologic and hematopoietic diseases
Humans
Leukemia, Erythroblastic, Acute - metabolism
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Medical sciences
Transcription, Genetic
Transfection
Tumor Cells, Cultured
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cited_by cdi_FETCH-LOGICAL-c375t-7ee93e8b4c61c3601c2e7cddc9c34acf0032454eb7cac6b6f4a76e1afd88cc73
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container_end_page 860
container_issue 4
container_start_page 855
container_title Blood
container_volume 77
creator DONOVAN-PELUSO, M
ACUTO, S
O'NEILL, D
HOM, A
MAGGIO, A
BANK, A
description We have constructed fusion genes comprised of gamma and beta globin elements and globin sequences linked to neomycin resistance (neoR) genes to define the cis acting sequences responsible for developmental stage-specific expression and induction of fetal globin genes in embryonic-fetal erythroleukemia K562 cells. The results indicate that the gamma promoter is required for proper initiation of transcription. However, the accumulation of gamma globin transcripts in response to hemin induction requires the additional presence of either gamma intervening sequence 2 or the 3' enhancer element of the beta globin gene. Thus, the gamma promoter may provide the elements for developmental stage-specific gene expression during fetal life. By contrast, the beta 3' enhancer is erythroid-specific but not developmental stage- or gene-specific.
doi_str_mv 10.1182/blood.V77.4.855.855
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ispartof Blood, 1991-02, Vol.77 (4), p.855-860
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source ScienceDirect Journals
subjects Biological and medical sciences
Cloning, Molecular
DNA, Recombinant
Embryo, Mammalian
Enhancer Elements, Genetic
Fetus - metabolism
Gene Expression Regulation
Genes
Globins - genetics
Hematologic and hematopoietic diseases
Humans
Leukemia, Erythroblastic, Acute - metabolism
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Medical sciences
Transcription, Genetic
Transfection
Tumor Cells, Cultured
title The β globin 3' enhancer element confers regulated expression on the human γ globin gene in the human embryonic-fetal erythroleukemia cell line K562
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