Production of polyclonal antibodies against the S and preS2 regions of woodchuck hepatitis virus: lack of detectable low glycosylated preS2 protein (GP33) in sera from infected animals

UPR 41 CNRS Recombinaisons Génétiques, Centre Hayem, Hôpital Saint-Louis, 75475 Paris Cedex 10, France Polyclonal antibodies directed against the preS2 and S domains of the woodchuck hepatitis virus (WHV) envelope proteins were prepared using synthetic peptides and fusion polypeptides as immunogens....

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Published in:Journal of general virology 1991-02, Vol.72 (2), p.421-425
Main Authors: Shamoon, Blanche-Marie, Kay, Alan, Dupont de Dinechin, Stephane, Galibert, Francis, Mandart, Elisabeth
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - immunology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Glycosylation
Hepadnaviridae - immunology
Hepatitis, Viral, Animal - immunology
Hepatitis, Viral, Animal - microbiology
Immunoblotting
Marmota
Microbiology
Precipitin Tests
Protein Precursors - blood
Protein Precursors - immunology
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Viral Envelope Proteins - blood
Viral Envelope Proteins - immunology
Virology
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Summary:UPR 41 CNRS Recombinaisons Génétiques, Centre Hayem, Hôpital Saint-Louis, 75475 Paris Cedex 10, France Polyclonal antibodies directed against the preS2 and S domains of the woodchuck hepatitis virus (WHV) envelope proteins were prepared using synthetic peptides and fusion polypeptides as immunogens. They were tested by immunoblotting and immunoprecipitation of infected woodchuck sera and lysates of a eukaryotic cell line expressing WHV envelope proteins. Only one anti-peptide serum directed against the preS2 domain was reactive with WHV envelope proteins, recognizing the preS2 and preS1 proteins by their preS2 epitopes. With recombinant fusion proteins we generated several anti-S sera, which recognized all envelope proteins, and anti-preS2 antisera, which recognized the preS proteins. Results obtained with our antisera showed that sera of infected woodchucks lack the low glycosylated form (GP33) of the preS2 protein, unlike human hepatitis B virus. Present address: UPR 2420 CNRS, Centre de Génétique Moléculaire, avenue de la Terrasse, 91198 Gif-sur-Yvette, France Received 4 July 1990; accepted 22 October 1990.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-72-2-421