Free-flow electrophoretic separation of Plasmodium berghei sporozoites

Sporozoites of the rodent malaria, Plasmodium berghei, were obtained from infected Anopheles stephensi by grinding mosquitoes, prepurifying the material in a discontinuous Hypaque gradient and further purifying by means of continuous free-flow electrophoresis. Bacteria, debris, mitochondria, mitopla...

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Bibliographic Details
Published in:The Journal of parasitology 1983-04, Vol.69 (2), p.360-367
Main Authors: Heidrich, H.G, Danforth, H.D, Leef, J.L, Beaudoin, R.L
Format: Article
Language:English
Subjects:
Animals
Anopheles - parasitology
Antigens
Electrophoresis
Experimental
Fluorescent Antibody Technique
Gels
human health
Malaria
Mitochondria
Molecular weight
Parasitism
Parasitology
Plasmodium berghei - analysis
Plasmodium berghei - cytology
Plasmodium berghei - isolation & purification
Polysaccharides - analysis
Proteins - analysis
Protozoa
Sporozoites
Vaccination
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Description
Summary:Sporozoites of the rodent malaria, Plasmodium berghei, were obtained from infected Anopheles stephensi by grinding mosquitoes, prepurifying the material in a discontinuous Hypaque gradient and further purifying by means of continuous free-flow electrophoresis. Bacteria, debris, mitochondria, mitoplasts, and other contaminants were removed in the electric field. The isolated sporozoites were morphologically intact and were positive in indirect immunofluorescence assay. They were infective to mice prior to and following free-flow electrophoretic separation. The surface of the sporozoites exhibited a polysaccharide-rich layer. The predominant surface protein labelled after surface iodination had a molecular weight between 42,000 and 46,000 daltons.
ISSN:0022-3395
1937-2345
DOI:10.2307/3281235