influence of cell type and culture medium on the in vitro cultivation of exoerythrocytic stages of Plasmodium berghei

Plasmodium berghei sporozoites successfully entered and developed into exoerythrocytic (EE) schizonts in variety of cell types cultured in vitro but segmentation and release of merozoites was only observed in human embryonic lung cells, EE development was generally not influenced by culture medium,...

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Bibliographic Details
Published in:The Journal of parasitology 1983-04, Vol.69 (2), p.346-352
Main Authors: Hollingdale, M.R, Leland, P, Leef, J.L, Beaudoin, R.L
Format: Article
Language:English
Subjects:
Animals
Blood
Cell culture techniques
Cell Line
Cells, Cultured - parasitology
Centrifugation
Cercopithecus aethiops
Cricetinae
Culture Media
Cultured cells
Embryonic cells
Hepatocytes
human health
Humans
In vitro Culture
Infections
Liver cells
Lungs
Mesocricetus
Mice
Neurons
Parasites
parasitology
Plasmodium berghei
Plasmodium berghei - growth & development
Protozoa
Rats
Rats, Inbred Strains
Saimiri
Sporozoites
Turkeys
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Summary:Plasmodium berghei sporozoites successfully entered and developed into exoerythrocytic (EE) schizonts in variety of cell types cultured in vitro but segmentation and release of merozoites was only observed in human embryonic lung cells, EE development was generally not influenced by culture medium, in vitro infectivity of sporozoites was unaffected by serum type used for isolation
ISSN:0022-3395
1937-2345
DOI:10.2307/3281232