The Effect of Monocytes in the Peripheral Blood CFU-C Assay System

• Published in: Blood 1983-07, Vol.62 (1), p.112-117
• Main Authors: To, L.B., Haylock, D.N., Juttner, C.A., Kimber, R.J.
• Format: Article
• Language: English
• Subjects: Animal cells; Biological and medical sciences; Cell Adhesion; Cell Communication; Cell cultures. Hybridization. Fusion; Colony-Forming Units Assay; Fundamental and applied biological sciences. Psychology; Humans; Lymphocytes - immunology; Molecular and cellular biology; Monocytes - cytology
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V62.1.112.112

The effect of cellular interactions in the in vitro assay of myeloid progenitor cells in peripheral blood (PB CFU-C) was investigated. Ficoll-Paque-separated peripheral blood mononuclear cells (PB MNC) from 7 healthy subjects were cultured at cell concentrations from 10 to 0.625 × 105 MNC/plate in doubling dilutions. The number of colonies per 106 lymphocytes plated (corrected colony count, CO was significantly higher when 2.5 × 105 or less PB MNC were cultured than when 5 or 10 × 105 cells were cultured. This decrease in CC when large numbers of cells were cultured was not present when the nonadherent cells only were cultured. The inhibition was reproduced when adherent cells were added back to the nonadherent cells. The inhibition appeared to be proportional to the number of monocytes present. A model depicting the role of monocytes in the PB CFU-C assay system is presented. The increased understanding of cellular interaction represents an important step towards the standardization of the PB CFU-C assay.