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The Effect of Monocytes in the Peripheral Blood CFU-C Assay System

The effect of cellular interactions in the in vitro assay of myeloid progenitor cells in peripheral blood (PB CFU-C) was investigated. Ficoll-Paque-separated peripheral blood mononuclear cells (PB MNC) from 7 healthy subjects were cultured at cell concentrations from 10 to 0.625 × 105 MNC/plate in d...

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Published in:	Blood 1983-07, Vol.62 (1), p.112-117
Main Authors:	To, L.B., Haylock, D.N., Juttner, C.A., Kimber, R.J.
Format:	Article
Language:	English
Subjects:	Animal cells Biological and medical sciences Cell Adhesion Cell Communication Cell cultures. Hybridization. Fusion Colony-Forming Units Assay Fundamental and applied biological sciences. Psychology Humans Lymphocytes - immunology Molecular and cellular biology Monocytes - cytology
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container_end_page	117
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container_title	Blood
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creator	To, L.B. Haylock, D.N. Juttner, C.A. Kimber, R.J.
description	The effect of cellular interactions in the in vitro assay of myeloid progenitor cells in peripheral blood (PB CFU-C) was investigated. Ficoll-Paque-separated peripheral blood mononuclear cells (PB MNC) from 7 healthy subjects were cultured at cell concentrations from 10 to 0.625 × 105 MNC/plate in doubling dilutions. The number of colonies per 106 lymphocytes plated (corrected colony count, CO was significantly higher when 2.5 × 105 or less PB MNC were cultured than when 5 or 10 × 105 cells were cultured. This decrease in CC when large numbers of cells were cultured was not present when the nonadherent cells only were cultured. The inhibition was reproduced when adherent cells were added back to the nonadherent cells. The inhibition appeared to be proportional to the number of monocytes present. A model depicting the role of monocytes in the PB CFU-C assay system is presented. The increased understanding of cellular interaction represents an important step towards the standardization of the PB CFU-C assay.
doi_str_mv	10.1182/blood.V62.1.112.112
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Ficoll-Paque-separated peripheral blood mononuclear cells (PB MNC) from 7 healthy subjects were cultured at cell concentrations from 10 to 0.625 × 105 MNC/plate in doubling dilutions. The number of colonies per 106 lymphocytes plated (corrected colony count, CO was significantly higher when 2.5 × 105 or less PB MNC were cultured than when 5 or 10 × 105 cells were cultured. This decrease in CC when large numbers of cells were cultured was not present when the nonadherent cells only were cultured. The inhibition was reproduced when adherent cells were added back to the nonadherent cells. The inhibition appeared to be proportional to the number of monocytes present. A model depicting the role of monocytes in the PB CFU-C assay system is presented. 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Ficoll-Paque-separated peripheral blood mononuclear cells (PB MNC) from 7 healthy subjects were cultured at cell concentrations from 10 to 0.625 × 105 MNC/plate in doubling dilutions. The number of colonies per 106 lymphocytes plated (corrected colony count, CO was significantly higher when 2.5 × 105 or less PB MNC were cultured than when 5 or 10 × 105 cells were cultured. This decrease in CC when large numbers of cells were cultured was not present when the nonadherent cells only were cultured. The inhibition was reproduced when adherent cells were added back to the nonadherent cells. The inhibition appeared to be proportional to the number of monocytes present. A model depicting the role of monocytes in the PB CFU-C assay system is presented. The increased understanding of cellular interaction represents an important step towards the standardization of the PB CFU-C assay.</description><subject>Animal cells</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion</subject><subject>Cell Communication</subject><subject>Cell cultures. Hybridization. Fusion</subject><subject>Colony-Forming Units Assay</subject><subject>Fundamental and applied biological sciences. 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Hybridization. Fusion</topic><topic>Colony-Forming Units Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Lymphocytes - immunology</topic><topic>Molecular and cellular biology</topic><topic>Monocytes - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>To, L.B.</creatorcontrib><creatorcontrib>Haylock, D.N.</creatorcontrib><creatorcontrib>Juttner, C.A.</creatorcontrib><creatorcontrib>Kimber, R.J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>To, L.B.</au><au>Haylock, D.N.</au><au>Juttner, C.A.</au><au>Kimber, R.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effect of Monocytes in the Peripheral Blood CFU-C Assay System</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1983-07</date><risdate>1983</risdate><volume>62</volume><issue>1</issue><spage>112</spage><epage>117</epage><pages>112-117</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>The effect of cellular interactions in the in vitro assay of myeloid progenitor cells in peripheral blood (PB CFU-C) was investigated. Ficoll-Paque-separated peripheral blood mononuclear cells (PB MNC) from 7 healthy subjects were cultured at cell concentrations from 10 to 0.625 × 105 MNC/plate in doubling dilutions. The number of colonies per 106 lymphocytes plated (corrected colony count, CO was significantly higher when 2.5 × 105 or less PB MNC were cultured than when 5 or 10 × 105 cells were cultured. This decrease in CC when large numbers of cells were cultured was not present when the nonadherent cells only were cultured. The inhibition was reproduced when adherent cells were added back to the nonadherent cells. The inhibition appeared to be proportional to the number of monocytes present. A model depicting the role of monocytes in the PB CFU-C assay system is presented. 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subjects	Animal cells Biological and medical sciences Cell Adhesion Cell Communication Cell cultures. Hybridization. Fusion Colony-Forming Units Assay Fundamental and applied biological sciences. Psychology Humans Lymphocytes - immunology Molecular and cellular biology Monocytes - cytology
title	The Effect of Monocytes in the Peripheral Blood CFU-C Assay System
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