Isolation of Group 2 Aerotolerant Campylobacter Species from Thai Children with Diarrhea

Campylobaeter species were isolated from 93 (15%) of 631 Thai children with diarrhea using the membrane filter technique on nonselective blood agar incubated at 377#x00B0;C. Campylobaeter jejuni was isolated from 62 (10%), Campylobaeter coli from 14 (2%), and atypical campylobacters from 17 (3%). Th...

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Bibliographic Details
Published in:The Journal of infectious diseases 1991-05, Vol.163 (5), p.1062-1067
Main Authors: Taylor, David N., Kiehlbauch, Julia A., Tee, Wee, Pitarangsi, Chittima, Echeverria, Peter
Format: Article
Language:English
Subjects:
Antibiotics
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Biochemistry
Biological and medical sciences
Blood
Campylobacter
Campylobacter - classification
Campylobacter - genetics
Campylobacter - isolation & purification
Campylobacter Infections - microbiology
Child, Preschool
Children
Diarrhea
Diarrhea - microbiology
DNA, Bacterial - analysis
Feces - microbiology
Genetic hybridization
Human bacterial diseases
Humans
Infant
Infections
Infectious diseases
Major Articles
Medical sciences
Nucleic Acid Hybridization
Pathogens
Phenotype
Thailand
Tropical medicine
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Summary:Campylobaeter species were isolated from 93 (15%) of 631 Thai children with diarrhea using the membrane filter technique on nonselective blood agar incubated at 377#x00B0;C. Campylobaeter jejuni was isolated from 62 (10%), Campylobaeter coli from 14 (2%), and atypical campylobacters from 17 (3%). The 17 atypical strains were first characterized biochemically and by dot blot DNA hybridization. Catalase-negative strains also were characterized byDNA hybridization and ribotype pattern. One strain was a catalase-negative “Campylobaeter upsaliensis” and another was a nitrate-negative Campylobaeter jejuni doylei. Fifteen isolates were aerotolerant strains most closely resembling Campylobaeter eryaerophila or “C. upsaliensis” by dot hybridization. These aerotolerantstrains, designated group 2 (“Campylobaeter butzleri”"),had ribotypes distinct from C. eryaerophila and have previously been shown to be related by DNA hybridization at the species level to the group 2 aerotolerant Campylobaeter type strain (D2686). Group 2 aerotolerant Campylobaeter were the atypical Campylobaeter species most frequently isolated from Thai children with diarrhea.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/163.5.1062