Simian Immunodeficiency Virus Infection of Macaques: End-Stage Disease Is Characterized by Widespread Distribution of Proviral DNA in Tissues

Four simian immunodeficiency virus (SIV)-infected macaques in the terminal stages of AIDS were studied. Easily detectable unintegrated proviral DNA was present in nearly every tissue (lymphoid and nonlymphoid) examined. SIV-specific immunohistochemistry revealed that macrophages or macrophage-like c...

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Bibliographic Details
Published in:The Journal of infectious diseases 1991-05, Vol.163 (5), p.976-988
Main Authors: Hirsch, Vanessa M., Zack, Philip M., Vogel, A. Peter, Johnson, Philip R.
Format: Article
Language:English
Subjects:
AIDS
AIDS/HIV
Amino Acid Sequence
Animals
Antigens, Viral - analysis
Base Sequence
Biological and medical sciences
Blotting, Southern
Brain - microbiology
Cell lines
Cloning, Molecular
DNA
DNA, Viral - analysis
DNA, Viral - chemistry
Ileum - microbiology
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunohistochemistry
Immunopathology
Infections
Kidney - microbiology
Lymph nodes
Lymph Nodes - microbiology
Lymphocytes
Lymphoid Tissue - microbiology
Macaca mulatta
Macaca nemestrina
Macrophages - microbiology
Major Articles
Medical sciences
Molecular Sequence Data
Polymerase Chain Reaction
Proviruses - genetics
Proviruses - immunology
Proviruses - isolation & purification
Simian Acquired Immunodeficiency Syndrome - microbiology
Simian Immunodeficiency Virus - genetics
Simian Immunodeficiency Virus - immunology
Simian Immunodeficiency Virus - isolation & purification
Spleen
Spleen cells
Virus Replication
Viruses
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Description
Summary:Four simian immunodeficiency virus (SIV)-infected macaques in the terminal stages of AIDS were studied. Easily detectable unintegrated proviral DNA was present in nearly every tissue (lymphoid and nonlymphoid) examined. SIV-specific immunohistochemistry revealed that macrophages or macrophage-like cells were the predominant reservoirs for SIV. High burdens of infectious SIV were also characteristic of end-stage disease. At the molecular level, sequence analyses of molecular clones derived directly from tissue demonstrated that SIV, like other RNA viruses, exists as a mixture of closely related genomes. Viruses derived by cocultivation seemed to be selected for their ability to grow in tissue culture and did not represent the wide spectrum of genotypes in tissues. These data indicate that SIV-induced immunodeficiency is a complex, multisystem disease and that SIV replication, in addition to impairing the immune system, may have direct deleterious effects on multiple organ systems.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/163.5.976