Use of New Synthetic Substrates for Assays of Cathepsin L and Cathepsin B

Efficient methods were developed for synthesizing synthetic substrates for assays of cathepsin B and cathepsin L. Several 2-naphthylamide compounds with a blocked NH2-terminus Suc-Tyr-Met-NA, β-Ala-Tyr-Met-NA, and D-Leu-Tyr-Met NA, were specific and sensitive substrates for cathepsin L and cathepsin...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1983-01, Vol.93 (4), p.1129-1135
Main Authors: KATUNUMA, Nobuhiko, TOWATARI, Takae, TAMAI, Masaharu, HANADA, Kazunori
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
assays
Biological and medical sciences
Cathepsin B
Cathepsin L
Cathepsins - analysis
Cysteine Endopeptidases
Endopeptidases
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Hydrolases
Hydrolysis
Male
Rats
Rats, Inbred Strains
Substrate Specificity
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Summary:Efficient methods were developed for synthesizing synthetic substrates for assays of cathepsin B and cathepsin L. Several 2-naphthylamide compounds with a blocked NH2-terminus Suc-Tyr-Met-NA, β-Ala-Tyr-Met-NA, and D-Leu-Tyr-Met NA, were specific and sensitive substrates for cathepsin L and cathepsin B; they were not specific for cathepsin L only, because all of them were also hydrolyzed by cathepsin B. Some kinetic constants for the hydrolyses of these three synthetic substrates by cathepsin B and cathepsin L are given.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a134238