Cloning and DNA sequence of the genes for two bacteriophage T5 tRNAsSer

Bacteriophage T5 BglII/HindIII DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNASer 1 and tRNASer 2) with anticodons UGA and GGA, r...

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Bibliographic Details
Published in:FEBS letters 1983-07, Vol.158 (1), p.123-127
Main Authors: Kryukov, V.M., Ksenzenko, V.N., Kaliman, A.V., Bayev, A.A.
Format: Article
Language:English
Subjects:
Bacteriophage T5
Base Sequence
Cloning, Molecular
DNA sequencing
DNA, Viral - genetics
Electrophoresis, Polyacrylamide Gel
Isoacceptor tRNA
Nucleic Acid Conformation
RNA, Transfer, Amino Acyl - genetics
RNA/DNA hydridization
T-Phages - genetics
tRNA gene
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Summary:Bacteriophage T5 BglII/HindIII DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNASer 1 and tRNASer 2) with anticodons UGA and GGA, respectively. The main unusual structural feature of these tRNAs is the presence of extra non‐basepaired nucleotides in the joinings of stem ‘b’ with stems ‘a’ and ‘c’.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(83)80690-5