Identification of p53 Gene Mutations in Bladder Cancers and Urine Samples

Although bladder cancers are very common, little is known about their molecular pathogenesis. In this study, invasive bladder cancers were evaluated for the presence of gene mutations in the p53 suppressor gene. Of 18 tumors evaluated, 11 (61 percent) were found to have genetic alterations of p53. T...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1991-05, Vol.252 (5006), p.706-709
Main Authors: Sidransky, David, Von Eschenbach, Andrew, Tsai, Yvonne C., Jones, Peter, Summerhayes, Ian, Marshall, Fray, Paul, Meera, Green, Pearl, Hamilton, Stanley R., Frost, Philip, Vogelstein, Bert
Format: Article
Language:English
Subjects:
Alleles
Base Sequence
Biological and medical sciences
Bladder cancer
Cancer
carcinoma
Cell lines
Chromosome Deletion
Chromosomes, Human, Pair 17
Diagnosis
Exons
Genes, p53
Genetic aspects
Genetic mutation
Genetic screening
Genetic testing
Genetics
Health aspects
Humans
Medical research
Medical sciences
Methods
MHC class II genes
Molecular Sequence Data
Mutation
Nephrology. Urinary tract diseases
Nucleic Acid Hybridization
Oligonucleotide Probes
p53 genes
Polymerase Chain Reaction
Tumor suppressor genes
Tumors
Tumors of the urinary system
Urinary bladder
Urinary Bladder Neoplasms - genetics
Urinary Bladder Neoplasms - urine
Urinary tract. Prostate gland
Urine
Urine - cytology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Although bladder cancers are very common, little is known about their molecular pathogenesis. In this study, invasive bladder cancers were evaluated for the presence of gene mutations in the p53 suppressor gene. Of 18 tumors evaluated, 11 (61 percent) were found to have genetic alterations of p53. The alterations included ten point mutations resulting in single amino acid substitutions, and one 24-base pair deletion. In all but one case, the mutations were associated with chromosome 17p allelic deletions, leaving the cells with only mutant forms of the p53 gene product. Through the use of the polymerase chain reaction and oligomer-specific hybridization, p53 mutations were identified in 1 to 7 percent of the cells within the urine sediment of each of three patients tested. The p53 mutations are the first genetic alterations demonstrated to occur in a high proportion of primary invasive bladder cancers. Detection of such mutations ex vivo has clinical implications for monitoring individuals whose tumor cells are shed extracorporeally.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2024123