Protection Against Malaria by Vaccination with Sporozoite Surface Protein 2 Plus CS Protein

The circumsporozoite (CS) protein has been the target for development of malaria sporozoite vaccines for a decade. However, immunization with subunit vaccines based on the CS protein has never given the complete protection found after immunization with irradiated sporozoites. BALB/c mice immunized w...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1991-05, Vol.252 (5006), p.715-718
Main Authors: Khusmith, Srisin, Charoenvit, Yupin, Kumar, Sanjai, Sedegah, Martha, Beaudoin, Richard L., Hoffman, Stephen L.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Monoclonal - immunology
Antibodies, Protozoan - immunology
Antigens
Antigens, Protozoan - genetics
Antigens, Protozoan - immunology
Applied microbiology
Biological and medical sciences
Cellular immunity
Cytotoxicity
Fundamental and applied biological sciences. Psychology
Immunization
Malaria
Malaria - prevention & control
Malaria vaccine
Malaria vaccines
Medical research
Mice
Mice, Inbred BALB C
Microbiology
Molecular Weight
Plasmodium yoelii - immunology
Protozoan Proteins - genetics
Protozoan Proteins - immunology
Protozoan Vaccines
Spleen cells
Sporozoites
T lymphocytes
T-Lymphocytes, Cytotoxic - immunology
T-Lymphocytes, Helper-Inducer - immunology
T-Lymphocytes, Regulatory - immunology
Transfection
Vaccination
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:The circumsporozoite (CS) protein has been the target for development of malaria sporozoite vaccines for a decade. However, immunization with subunit vaccines based on the CS protein has never given the complete protection found after immunization with irradiated sporozoites. BALB/c mice immunized with irradiated Plasmodium yoelii sporozoites produced antibodies and cytotoxic T cells against a 140-kilodalton protein, sporozoite surface protein 2 (SSP2). Mice immunized with P815 cells that had been transfected with either SSP2 or CS genes were partially protected, and those immunized with a mixture of SSP2 and CS transfectants were completely protected against malaria. These studies emphasize the importance of vaccine delivery systems in achieving protection and define a multi-antigen sporozoite vaccine.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1827210