Simplified determination of antipyrine clearance by liquid chromatography of a microsample of saliva or plasma

We describe a simplified and rapid liquid chromatographic determination of antipyrine clearance (CLAP) calculated from peak height ratios of drug/internal standard. Saliva or plasma was collected 24 hr after the oral administration of 1 g of antipyrine to the subject. A 25-microliter aliquot of the...

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Bibliographic Details
Published in:Pharmaceutical research 1991-02, Vol.8 (2), p.269-272
Main Authors: ADNAN EL-YAZIGI, RAINES, D. A, HATIM ALI, SIECK, J, ERNST, P, DOSSING, M
Format: Article
Language:English
Subjects:
Analysis
Antipyrine - blood
Antipyrine - pharmacokinetics
Biological and medical sciences
Chromatography, Liquid
General pharmacology
Humans
Medical sciences
Metabolic Clearance Rate
Pharmacology. Drug treatments
Saliva - metabolism
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Summary:We describe a simplified and rapid liquid chromatographic determination of antipyrine clearance (CLAP) calculated from peak height ratios of drug/internal standard. Saliva or plasma was collected 24 hr after the oral administration of 1 g of antipyrine to the subject. A 25-microliter aliquot of the sample is deproteinized with acetonitrile containing 3-nitrophenol (internal standard) and injected into a radial compression module equipped with a 10-micron, 8 mm x 10-cm C18 cartridge, using a 0.025 M aqueous solution of sodium acetate and acetonitrile (88.5:11.5). The minimum measurable concentration was 0.2 microgram/ml. The obtained CLAP values in five healthy subjects and five patients with chronic liver disease coincided well (r greater than 0.9994) with those generated by the use of an established method. The antipyrine clearance in the healthy subjects ranged from 2.203 to 5.721 liters/hr, while in patients with chronic liver disease it was significantly (P less than 0.0027) less (range, 0.544 to 1.103 liter/hr). We also determined antipyrine clearance in two of these subjects given lower doses of this drug and found that the dose has no significant impact on this parameter.
ISSN:0724-8741
1573-904X
DOI:10.1023/A:1015816825920