Multipolar mitotic cells in the human cell line NHIK 3025 following treatment with the mitotic inhibitor NY 4137

Treatment of human NHIK 3025 cells during interphase with the mitotic inhibitor 2-(2-thenyl) sulfonyl-5-bromopyrimidine (NY 4137) induced the formation of multipolar mitotic cells. A 4 h pulse treatment with 0.010 mM NY 4137 induced the formation of such cells regardless of whether treatment occurre...

Full description

Saved in:
Bibliographic Details
Published in:Investigational new drugs 1991-02, Vol.9 (1), p.19-28
Main Authors: HOLM JUUL, M. E, DORNISH, J. M, SCHWARZE, P. E, PETTERSEN, E. O, OFTEBRO, R
Format: Article
Language:English
Subjects:
Antineoplastic agents
Antineoplastic Agents - pharmacology
Biological and medical sciences
Cell Division
Cell Separation
DNA - metabolism
Female
Flow Cytometry
General aspects
Humans
Interphase - drug effects
Medical sciences
Mitosis - drug effects
Pharmacology. Drug treatments
Proteins - metabolism
Pyrimidines - pharmacology
Tumor Cells, Cultured - drug effects
Uterine Cervical Neoplasms - pathology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Treatment of human NHIK 3025 cells during interphase with the mitotic inhibitor 2-(2-thenyl) sulfonyl-5-bromopyrimidine (NY 4137) induced the formation of multipolar mitotic cells. A 4 h pulse treatment with 0.010 mM NY 4137 induced the formation of such cells regardless of whether treatment occurred in G1 or S phase. Approximately 35% of the cells originating from such multipolar mitotic divisions had less-than-normal G1 DNA content. Recordings of the DNA content of these small cells by means of flow cytometry showed that the amount of DNA per cell varied randomly. Subpopulations were separated by centrifugal elutriation on the basis of differences in cellular volume. Simultaneous two-parametric recordings of DNA versus total cellular protein showed that even though cells contained an amount of DNA less than G1 cells, there was a proportionality between DNA content and protein content despite variations in cell size.
ISSN:0167-6997
1573-0646
DOI:10.1007/BF00194540