Plasma Protein and Apolipoprotein Synthesis by Human Yolk SAC Carcinoma Cells in vitro

Three human yolk sac carcinoma cell lines were characterized for the expression of several markers. Each of the cell lines expressed alpha-fetoprotein, without detectable levels of chorionic gonadotropin, and the level of alpha-fetoprotein expression increased dramatically when the cultures were hel...

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Bibliographic Details
Published in:In Vitro Cellular & Developmental Biology - Animal 1991-03, Vol.27A (3), p.205-210
Main Authors: Lanford, Robert E., Bronson, David L., Larry E. Estlack, Frank H. Wians, Jr
Format: Article
Language:English
Subjects:
alpha-Fetoproteins - metabolism
Analytical, structural and metabolic biochemistry
Apolipoproteins - metabolism
Biological and medical sciences
Blood proteins
Blood Proteins - immunology
Blood Proteins - metabolism
Carcinoma
Cell lines
Chorionic Gonadotropin - metabolism
Cultured cells
Female
Flasks
Fundamental and applied biological sciences. Psychology
Germ cells
Humans
Immunoprecipitation
In Vitro Techniques
Lipoproteins
Mesonephroma - metabolism
Ovarian Neoplasms - metabolism
Precipitin Tests
Proteins
Tumor Cells, Cultured
Tumors
Yolk sac
Yolk Sac - metabolism
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Summary:Three human yolk sac carcinoma cell lines were characterized for the expression of several markers. Each of the cell lines expressed alpha-fetoprotein, without detectable levels of chorionic gonadotropin, and the level of alpha-fetoprotein expression increased dramatically when the cultures were held without passage for extended periods. The secretion of a number of plasma proteins was documented by metabolic labeling, immunoprecipitation, and gel analysis. The major plasma proteins detected were alpha-1-antitrypsin, alpha-fetoprotein, transthyretin, β-2 microglobulin, and plasminogen, with lower levels of transferrin and complement C4 released. Apolipoproteins B, E, and A1 were secreted in high levels as well and were found in the form of lipoprotein particles. Time course experiments on the synthesis of apolipoproteins E and A1 indicated that, as with alpha-fetoprotein, the level of synthesis increased substantially when the cultures were held without passage. The results indicate that these yolk sac carcinoma cells display a protein expression profile similar to that observed for the human yolk sac, and the possibility that the cells may have the potential to differentiate is discussed.
ISSN:0883-8364
2327-431X
1543-706X
DOI:10.1007/BF02630917