Phycoerythrins of marine unicellular cyanobacteria. II. Characterization of phycobiliproteins with unusually high phycourobilin content

• Published in: The Journal of biological chemistry 1991-05, Vol.266 (15), p.9528-9534
• Main Authors: SWANSON, R. V, ONG, L. J, WILBANKS, S. M, GLAZER, A. N
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chromatography, High Pressure Liquid; Cyanobacteria - metabolism; cyanophyta; espectrometria; Fundamental and applied biological sciences. Psychology; Heterocyclic compounds, pigments; Light-Harvesting Protein Complexes; Molecular Sequence Data; Other biological molecules; Phycobilins; Phycoerythrin - analysis; Phycoerythrin - metabolism; pigment; pigmentos; pigments; Pigments, Biological; Plant Proteins - chemistry; purificacion; purification; Sequence Homology, Nucleic Acid; spectrometrie; spectrometry; Spectrometry, Fluorescence; Tetrapyrrolic pigments; Urobilin - analogs & derivatives
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)92852-8

A survey of marine unicellular cyanobacterial strains for phycobiliproteins with high phycourobilin (PUB) content led to a detailed investigation of Synechocystis sp. WH8501. The phycobiliproteins of this strain were purified and characterized with respect to their bilin composition and attachment sites. Amino-terminal sequences were determined for the alpha and beta subunits of the phycocyanin and the major and minor phycoerythrins. The amino acid sequences around the attachment sites of all bilin prosthetic groups of the phycocyanin and of the minor phycoerythrin were also determined. The phycocyanin from this strain carries a single PUB on the alpha subunit and two phycocyanobilins on the beta subunit. It is the only phycocyanin known to carry a PUB chromophore. The native protein, isolated in the (alpha beta)2 aggregation state, displays absorption maxima at 490 and 592 nm. Excitation at 470 nm, absorbed almost exclusively by PUB, leads to emission at 644 nm from phycocyanobilin. The major and minor phycoerythrins from strain WH8501 each carry five bilins per alpha beta unit, four PUBs and one phycoerythrobilin. Spectroscopic properties determine that the PUB groups function as energy donors to the sole phycoerythrobilin. Analysis of the bilin peptides unambiguously identifies the phycoerythrobilin at position beta-82 (residue numbering assigned by homology with B-phycoerythrin; Sidler, W., Kumpf, B., Suter, F., Klotz, A.V., Glazer, A.N., and Zuber, H. (1989) Biol. Chem. Hoppe-Seyler 370, 115-124) as the terminal energy acceptor in phycoerythrins.