Transmission of Retroviral Infection by Transfusion of Seronegative Blood in Nonhuman Primates

Techniques such as polyclonal B cell activation with pokeweed mitogen (PWM) and polymerase chain reaction (PCR) analysis have documented the existence of simian immunodeficiency virus(SIV)-and human immunodeficiency virus type I-seronegattve but infected humansand nonhuman primates. To establish whe...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 1991-06, Vol.163 (6), p.1223-1228
Main Authors: Jehuda-Cehen, Tamar, Powell, Jonathan D., Sell, Kenneth W., Villinger, Francois, Lockwood, Ellen, McClure, Harold M., Ahmed-Ansari, A.
Format: Article
Language:English
Subjects:
AIDS/HIV
Animals
Antibodies
Antibodies, Viral - blood
B lymphocytes
Biological and medical sciences
Blood
Blood Transfusion
Blotting, Western
Cercopithecidae
Cultured cells
DNA, Viral - analysis
Enzyme-Linked Immunosorbent Assay
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Infections
Lymphocyte Activation
Macaca mulatta
Macaca nemestrina
Major Articles
Medical sciences
Polymerase Chain Reaction
Primates
Reactivity
Simian Acquired Immunodeficiency Syndrome - transmission
Simian Immunodeficiency Virus - genetics
Simian Immunodeficiency Virus - immunology
Simian Immunodeficiency Virus - physiology
Virus Replication
Viruses
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Techniques such as polyclonal B cell activation with pokeweed mitogen (PWM) and polymerase chain reaction (PCR) analysis have documented the existence of simian immunodeficiency virus(SIV)-and human immunodeficiency virus type I-seronegattve but infected humansand nonhuman primates. To establish whether blood from suchseronegative but PWM- and PCR-positive monkeys can transmit infection, naive macaques were transfused with whole blood (n =2) or cultured cells and supernatant fluid (n =2) from two seronegative but PWM- and PCR-positive sooty mangabeys. Aftertransfusion, threeofthefourrecipients seroconverted, and peripheral blood mononuclear cells from all four recipients secreted SIV-reactive antibodies upon polyclonal activation in vitroand were SIV-positive by PCR that used highly specific gag primer pairs and probe. In addition, CD8+ cells from all four recipients markedly inhibited replication of SIV in autologous cells in vitro. These data suggest caution in the sole use of serologic tests forthe detection ofretroviral infection and document the ability ofsuchblood samples to transmit infection.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/163.6.1223