Rapid characterization of Mycobacterium fortuitum-chelonei complex by restriction fragment length polymorphism of ribosomal RNA genes

Using labelled, gamma-32P rRNA of mycobacteria as a probe restriction fragment length polymorphism (RFLP) of rRNA genes of strains belonging to the Mycobacterium fortuitum-chelonei complex was analysed. Each DNA sample was cleaved with EcoRI restriction endonuclease, the fragments were separated by...

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Bibliographic Details
Published in:FEMS microbiology letters 1991-01, Vol.61 (2-3), p.205-208
Main Authors: Kanaujia, G V, Katoch, V M, Shivannavar, C T, Sharma, V D, Patil, M A
Format: Article
Language:English
Subjects:
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Genes, Bacterial
Mycobacterium - classification
Mycobacterium - genetics
Mycobacterium - isolation & purification
Nucleic Acid Hybridization
Polymorphism, Restriction Fragment Length
Restriction Mapping
RNA Probes
RNA, Ribosomal - genetics
RNA, Ribosomal - isolation & purification
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Summary:Using labelled, gamma-32P rRNA of mycobacteria as a probe restriction fragment length polymorphism (RFLP) of rRNA genes of strains belonging to the Mycobacterium fortuitum-chelonei complex was analysed. Each DNA sample was cleaved with EcoRI restriction endonuclease, the fragments were separated by agarose gel electrophoresis and transferred to nitrocellulose membrane. Fragments of DNA containing rRNA genes were identified by hybridization with gamma-32P-labelled rRNA. Patterns were found to be species specific and both the species were distinguishable from each other. Results indicate that this approach can be used for rapid genomic characterization of the Mycobacterium fortuitum-chelonei complex.
ISSN:0378-1097
DOI:10.1016/0378-1097(91)90552-L