Molecular properties of the slow inward calcium channel. Molecular weight determinations by radiation inactivation and covalent affinity labeling

The slow inward calcium channel, identified by physiologic and pharmacologic responses and [3H]nitrendipine-specific binding, has been characterized by radiation inactivation and covalent affinity labeling. Target size analysis of guinea pig ileum longitudinal smooth muscle membranes indicates a mol...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1983-08, Vol.258 (15), p.9344-9348
Main Authors: Venter, J C, Fraser, C M, Schaber, J S, Jung, C Y, Bolger, G, Triggle, D J
Format: Article
Language:English
Subjects:
Affinity Labels - metabolism
Animals
Biological and medical sciences
Calcium - metabolism
Cell physiology
Dihydropyridines
Fundamental and applied biological sciences. Psychology
Guinea Pigs
Ion Channels - metabolism
Ion Channels - radiation effects
Membrane and intracellular transports
Methods
Molecular and cellular biology
Molecular Weight
Muscles - metabolism
Nifedipine - analogs & derivatives
Nifedipine - metabolism
Nitrendipine
Pyridines - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The slow inward calcium channel, identified by physiologic and pharmacologic responses and [3H]nitrendipine-specific binding, has been characterized by radiation inactivation and covalent affinity labeling. Target size analysis of guinea pig ileum longitudinal smooth muscle membranes indicates a molecular weight of 278,000 for the calcium channel. An affinity label analog of nifedipine and nitrendipine, 2,6-dimethyl-3,5-dicarbomethoxy-4-(2-isothiocyanatophenyl)-1,4-dihydropyridine, was found to inhibit the calcium channel by a covalent interaction with a protein subunit (Mr = 45,000) of the calcium channel.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)44673-4