Morphological Evaluation of Pneumocystis carinii after Extraction from Infected Lung

Changes in Pneumocystis carinii induced by the extraction of the parasite from rabbit lung have been investigated. Samples obtained using 4 extraction methods were evaluated by light and transmission electron microscopy. Light microscopic evaluation was insufficient to give a measure of the P. carin...

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Bibliographic Details
Published in:The Journal of parasitology 1991-06, Vol.77 (3), p.449-453
Main Authors: Soulez, Benoit, Dei-Cas, Eduardo, Palluault, François, Camus, Daniel
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cysts
Fundamental and applied biological sciences. Psychology
General aspects and techniques
Invertebrates
Lung - parasitology
Lungs
Microscopy, Electron
Morphology and Ultrastructure
Parasite hosts
Parasites
Parasitism
Parasitology
Pneumocystis - growth & development
Pneumocystis - ultrastructure
Pneumocystis carinii
Pneumonia, Pneumocystis - parasitology
Protozoa
Rabbits
Specimens
Trophozoites
Viability
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Summary:Changes in Pneumocystis carinii induced by the extraction of the parasite from rabbit lung have been investigated. Samples obtained using 4 extraction methods were evaluated by light and transmission electron microscopy. Light microscopic evaluation was insufficient to give a measure of the P. carinii viability or to detect parasitic cellular alterations. In contrast, ultrastructural evaluation provided information on host and P. carinii cell integrity, which is a critical condition for viability. None of the tested methods was ideal. How thoroughly and in what shape P. carinii need to be extracted from tissues will determine which extraction technique is of best use.
ISSN:0022-3395
1937-2345
DOI:10.2307/3283134