Membrane-bound carbonic anhydrase CA IV in the human kidney

The distribution of membrane‐bound carbonic anhydrase, CA IV, was studied in human kidneys by an indirect immunoperoxidase method using a rabbit polyclonal antibody directed against human kidney CA IV. Clear staining of CA IV was found in the apical cell borders of some cells in the cortical and med...

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Bibliographic Details
Published in:Acta physiologica Scandinavica 1991-02, Vol.141 (2), p.231-234
Main Authors: LÖNNERHOLM, G., WISTRAND, P. J.
Format: Article
Language:English
Subjects:
Biocarbonate secretion
Biological and medical sciences
carbobnic anhydrase
Carbonic Anhydrases - analysis
Cell Membrane - enzymology
Cell Membrane - ultrastructure
cell membranes
Fundamental and applied biological sciences. Psychology
Humans
Immunohistochemistry
kidney
Kidney - cytology
Kidney - enzymology
Kidney - ultrastructure
Kidney Tubules, Collecting - cytology
Kidney Tubules, Collecting - enzymology
Kidney Tubules, Collecting - ultrastructure
Kidney Tubules, Proximal - cytology
Kidney Tubules, Proximal - enzymology
Kidney Tubules, Proximal - ultrastructure
Microvilli - enzymology
Microvilli - ultrastructure
plasma membranes
renal tubular acidosis
Vertebrates: digestive system
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Summary:The distribution of membrane‐bound carbonic anhydrase, CA IV, was studied in human kidneys by an indirect immunoperoxidase method using a rabbit polyclonal antibody directed against human kidney CA IV. Clear staining of CA IV was found in the apical cell borders of some cells in the cortical and medullary segments of the collecting ducts, presumably the A type of intercalated cells. Weak staining for CA IV was located in the interior of a number of collecting duct cells and in the basolateral regions of the proximal convoluted tubules. However, no staining was found in the brush border of the same tubules. This is a surprising finding, since evidence for carbonic anhydrase activity has been found biochemically and histochemically both in isolated brush‐border and baso‐lateral membranes. Further work is needed to clarify this matter. The endothelium of the peritubular capillaries also stained for CA IV.
ISSN:0001-6772
1365-201X
DOI:10.1111/j.1748-1716.1991.tb09072.x